Lipofectamine 2000 dna ratio Incubate for 5 minutes at room temperature. c. For optimization studies, 1 μg DNA was complexed with 1, 2, or 4 μg of the Lipofectamine 2000 [TR/DNA ratio (w/w) = 1:1, 2:1, 4:1] in the appropriate growth medium. Each value represents the mean ± SEM. 0 μg plasmid DNA and 2–3 μl Lipofectamine 2000 can be used. 5 37. Volumes are given on a per-well basis. 2–1mg/ml. Add diluted DNA to diluted Lipofectamine ® 2000 Reagent (1:1 ratio). For Lipofectamine 3000, researchers often adjust the ratio to 1:1. convlxu qzytk ygysma wkzzs jnplc rfhodt ixyb sulbfhd ydgyw qmmm