Lenticrispr v2 sequencing primer Optional: run 200ng of the purified vector on a gel to verify digestion. No. Cloning guides to lentiCRISPR v2 Created Date: 5/25/2025 4:44:04 PM The editing activity of sgRNA can also be determined by sequencing the PCR product generated using surveyor primers. Zhang lab lentiviral transfer plasmid, also known as pLentiCRISPR v2, for CRISPR/Cas9 genome editing of mammalian cells with a single guide RNA (sgRNA). Brett Stringer's lab contains the insert P2A-puro and is published in Sci Rep. lentiCRISPR v2 was similarly digested and the larger fragment gel-purified. 1 5’(5'- GACTATCATATGCTTACCGT-3'). Protocol search Table lists primer name, sequence, length, binding site location, and direction. 8 kb，测序引物为hEF1aprom-F/WPRE-R。 Plasmid lentiCRISPR - EGFP sgRNA 1 from Dr. Here, we demonstrate the utility of sequentially performed CRISPR knockout screens that use an existing library to explore a biological question across the human genome, and then the remaining cells are used to examine each Dec 15, 2023 · Moreover, different libraries use different backbones and systems for the transduction of sgRNAs.
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